Talk by Karen Perronet from the Optical Institute, Paris

Mammalian protein synthesis studied by single-molecule fluorecscence microscopy

Protein synthesis is a complex multistep process involving many factors that need to interact in a coordinated manner to properly translate the messenger RNA. As translating ribosomes cannot be synchronized over many elongation cycles, single-molecule studies have been introduced to bring a deeper understanding of prokaryotic translation dynamics. Extending this approach to eukaryotic translation is very appealing, but initiation and specific labeling of the ribosomes are much more complicated. We use a noncanonical translation initiation based on internal ribosome entry sites (IRES), and we monitor the passage of individual, unmodified mammalian ribosomes at specific fluorescent milestones along mRNA. We explore initiation by three types of IRES, and show that they all strongly limit the rate of the first elongation steps compared to the following ones, suggesting that those first elongation cycles do not correspond to a canonical elongation. This new system opens the possibility of studying both IRES-mediated initiation and elongation kinetics of eukaryotic translation and will undoubtedly be a valuable tool to investigate the role of translation machinery modifications in human diseases.

Host: Dr David Dulin

OICE successfully supports international research co-operation led by FAU scientists

Researchers from four different countries co-ordinated by Dr. Nicole Tegtmeyer working at the Chair of Microbiology led by Prof. Stefffen Backert discovered how Helicobacter pylori employs a unique basolateral
type IV secretion mechanism for CagA delivery. OICE is quite happy to have been able to contribute to this work, which led to a publication in Cell Host & Microbe. Furthermore the Friedrich-Alexander University thought this to be worth an official press release, too. You find the full text article online at Cell Press.

Microscopy V-OICE of Erlangen Symposium ahead

Robert Prevedel - EMBL confirmed Keynote Speaker

Date is October 26th at OICE and opening will be at 1 p.m.. Dr. Prevedel is a young EMBL group leader who is interested in advanced optical techniques for deep tissue microscopy. There will be brief talks on the different imaging modalities at OICE from members of staff and from different research groups. A poster contest with one abstract chosen for oral presentation and a poster prize. Of course all instruments will be running and demos will be given by members of staff. The symposium will conclude with the poster session accompanied by fingerfood & drinks and the announcement of the poster prize winner at around 7 p.m.. DOWNLOAD PDF

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Major system extensions scheduled

We are happy to inform that there will be some nice extensions to the following systems in very near future:

  • Z-drift compensation unit for STED and RESOLFT with our Abberior super-resolution system.
  • Upgrade to Definite Focus 2 from the wacky first release on our Zeiss Spinning Disc Microscope.
  • Extension of the Zeiss / Spectra-Physics intra-vital microscope with four new detectors for simultaneous image acquisition (4 NDDs consisting of 2 MA-PMT and 2 GaAsP detectors on the left side-port of the LSM 880 NLO system.

OICE with Plenary Talk at MMC 2017

"Optical clearing of plant tissue without fixation  for intra-vital imaging of virus infection in N. benthamiana leaves by multi-photon microscopy" is an emerging new technique pushed by scientists from OICE and the group of B. Krenz (Plant Biochemistry). This was of sufficient interest to the reviewers to give us (Ralf Palmisano) the opportunity to talk to the audience at MMC2017 in Manchester. Furthermore we (Philipp Tripal) also presented a poster on "Extending the insight into in-vitro human cornea by using multi-photon microscopy combining fluorescence with SHG imaging reaching out for more than 2 mm of penetration depth" in co-operation with the Fuchsluger group from the Opthalmic medical department.

OICE with Plenary Talk at ELMI 2017

OICE was picked to give a plenary lecture at ELMI (European Light Microscopy Initiative). Member of staff Philipp Tripal stood strong and stable and of course he shone! Philipp talked about "Three dimensional live cell imaging of intestinal organoid structures by spinning disc microscopy". This is a very challenging emerging new technique and a great co-operation with Prof. Becker and members of his Division of Molecular Gastroenterology, Department of Medicine 1. Together we are creating fascinating new protocols to establish long-term observation of multicellular organoids of different origin. This will reveal new insights. Full abstract can be found here.

Roofing Ceremony of New Research Building

On Thursday 11th the roofing ceremony of the IZNF Research Building took place. A great opening by trumpets was followed by welcoming speeches from Dieter Maußner, Head of the State Building Authority, the Bavarian Home Secretary Joachim Herrmann, the Parliamentary Secretary from the BMBF Berlin Stefan Müller, followed by Erlangens Town Mayor Dr. Florian Janik, the FAU President Prof. Joachim Hornegger and the Chair of the Excellence Cluster Engineering of Advanced Materials (EAM) Prof. Peter Wasserscheid. The ceremony was concluded as it is the long lasting tradition by the site manager Holger Kessler and his ganger. When the toasts were given and the well-wishing, including the breakage of the glasses (thrown down from the second floor...) had happened we all enjoyed the traditional feast together with the builders, craftsmen and workers form the construction site. They did a tremendous job so far, as the building process is fully within schedule by now. More images can be seen here.

First official site visit at the new research building

On March 29th we had the pleasure to get the first tour through the almost fully erected new research building. It is very impressive to see this place now grow fast and we were told, that the builders are only a few days behind schedule due to some frosty days, while it was planned to process some concrete.

Prof. Erdmann Spieker from CENEM and Dr. Ralf Palmisano (OICE) standing on the first floor of what is to become Prof. Spiekers office, with the site to become Dr. Palmisano's office in the background. Obviously both are looking forward to start co-operations between the electron and the light microscopy unit.

1st OPEN DAY Great Success

This was it. The 1st open day @ OICE. More than 60 participants made their way to the keynote lecture given by Dr. Ulrike Endesfelder, from the MPI Terrestrial Microbiology talking about Single Molecule Localisation Microscopy. Dr. Endesfelder gave us the pleasure to share latest yet unpublished fascinating data from her group. That was appreciated very well from the audience. This was followed by 5 inspiring short talks. The demo sessions were also very well looked after, before people gathered for some nice food and drinks to talk.

Intravital Zeiss 880 NLO Airyscan with DeepSee Laser Arrived

We are happy to announce that the one of its kind set-up for intravital imaging has arrived and OICE will deliver courses for our users quite soon. It is a custom build setup with Carl Zeiss and Spectra-Physics. The basis is a Zeiss 880 Axioimager fixed stage NLO system, with an Airyscan detector and non-descanned detector directly built into the turret at the backplane opening of the objective lens. This you will not loose a single photon emerging from your intravital samples while using MP excitation. The DeepSee laser delivers a tunable range from 680 up to 1300 nm and a fixed wavelength at 1040 nm. This will certainly enable FAU and adjacent user to perform uncrompomised deep tissue and intravital imaging.

Ground-Breaking Ceremony for New Research Building

On May 6th ground-breaking ceremony took place for the new research builidng of the Interdsciplinary Centre for Nanostructured Surfaces. Representatives from the Friedrich-Alexander University, the City of Erlangen, the State of Bavaria and the Ministry of Science and Education from the German Goverment made their way to celebrate. With a bright blue sky and a warm summer like breeze the representatives and honorary guests were all smiles. The combined effort of 40 million Euro we lead to a unique assembly of different approache on nano structures and their develepoment. OICE will be the major permanent user of this new building covering approximately one quarter of the floor space. More information in german are accessible here. We are absolutly delighted and are looking forward to the second half of 2018 when we hopefully will be moving into our then new home.

Ground-Breaking Ceremony View of contruction site President of FAU Prof. J. Horrnegger The inevitable talks The inevitable talks

First Intra-European Imaging Workshop hosted at OICE

As OICE is part of the ETN MAGICBULLET, consortium within the Horizon 2020 MSCA excellence initiative, we are happy to have had the first imaging workshop for the ESRs from four different european countries within the EU. The workshop took place March 7th - 11th.

ESRs from the Magicbullet consortium


Some obviously happy ESRs from the consortium enjoyed a packed week of imaging and sunshine!

New Cell Line for RESOLFT Super Resolution Imaging Arrived

We are happy to announce that OICE can offer to make use of a phantastic cell line for your Resolft super-resolution imaging that is stable transformed with Vimentin-rsEGFP2. This is agenerous gift by Prof. Stefan Jakobs from the Max Planck Institute for Biophysical Chemistry in Goettingen. If you are interested in using it at OICE, please contact a member of staff.